曾星 杨明 黄羽 章国来 广州中医药大学第二附属医院中心实验室，广州 510120 广州中医药大学学报 1999 0 16 1


    关键词：卡介苗；信号肽类；白细胞介素2；膀胱肿瘤/治疗 期刊 gzzyydxxb 0 72??74 新学科与新技术 fur -->


    

摘要：采用聚合酶链反应及基因工程技术，克隆卡介苗(BCG)的主要分泌抗原Ag85B基因的5’端第94～211的核苷酸的信号肽序列和构建重组人 IL-2 穿梭表达质粒，并用酶联免疫吸附法(ELISA)鉴定外源基因在BCG中的表达。结果表明，构建的BCG重组体能表达和分泌人 IL-2。此将试用于临床治疗膀胱癌病人。

    中图分类号：Q784 文献标识码：A

    文章编号：1007-3213(1999)01-0072-04

Construction of Recombinant Mycobacterium bovis BCG and Expression of

    the Foreign Gene in BCG

ZENG Xing, YANG Ming, HUANG Yu, ZHANG Guo_lai

    (Central Laboratory, the Second Affiliated Hospital of Guangzhou University of TCM,Guangzhou 510120,China)

Abstract:Polymerase chain reaction and genetic engineering technique were used to clone the signal peptide sequence (position:94 to 211) of antigen 85-B in Mycobacterium bovis BCG and construct the recombinant E.coli-mycobacteria shuttle plasmoid of human interleukin-2(IL-2).Expression of the foreign gene in BCG was detected by enzyme linked immunosorbent assay. The results suggest that the constructed recombinant BCG(rBCG) strain produce and secrete human IL-2 and it will be used for the treatment of superficial bladder cancer.

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